Suicide in Serial Experiments Lain

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Suicide is a common occurrence in Lain's world. The first suicide the viewer sees is the death of Yomoda Chisa by jumping from a building at the very beginning of Layer 01. The most common reason for doing so is to "abandon the flesh" and pursue an existence on the Wired alone.

After the members of the Knights of the Eastern Calculus are exposed in Layer 10, they all commit suicide. They all seem to have been prepared for such a course of action.

Masami Eiri appears to have committed suicide by jumping in front of a train in order to become God in the Wired.

It is debatable whether Lain herself can be considered to have committed suicide at the end of the anime. She erases her own existence, including her past, in order to fix all the damage she has dealt to the world, but she still clearly exists in the Wired and, at one point, manifests in front of a grown-up Alice.

In the game, Lain commits suicide by shooting herself through her mouth.

The concept of "abandoning the flesh" is popular in the Lain fandom and is something of a meme or inside joke between fans. On Mebious.co.uk, for example, people occasionally encourage each other to ascend to the upper level of reality, the Wired, and post about it after they have succeeded.